ANTIFUNGAL AND INSECTICIDAL ACTIVITIES OF ESSENTIAL OILS OF CHRYSANTHEMUM CORONARIUM AND ACHILLEA MILLEFOLIUM AGAINST CITRUS FRUITS PESTS AND DISEASES

Authors

  • Benazzouz Fatima Zohra Laboratory for Ecology and Management of Natural Ecosystems (LECGEN), Department of Ecology and Environment, Faculty of Natural and Life Sciences and Earth and Universe Sciences, Abou Bekr Belkaid University
  • Kholkhal Wahiba Laboratory for Ecology and Management of Natural Ecosystems (LECGEN), Department of Ecology and Environment, Faculty of Natural and Life Sciences and Earth and Universe Sciences, Abou Bekr Belkaid University
  • Dib Mohammed El Amine Natural and Bioactive Substances Laboratory (LASNABIO), Department of Chemistry, Faculty of Sciences, Abou Bekr Belkaid University https://orcid.org/0000-0003-4612-5303
  • Bounouira Yassine Faculty of Natural and Life Sciences, Department of Agriculture, Forestry and Environmental Sciences, Tissemsilt University https://orcid.org/0000-0003-1698-5652
  • Gaouar Benyelles Nassira Laboratory for Ecology and Management of Natural Ecosystems (LECGEN), Department of Ecology and Environment, Faculty of Natural and Life Sciences and Earth and Universe Sciences, Abou Bekr Belkaid University https://orcid.org/0009-0001-1580-1965

DOI:

https://doi.org/10.18623/rvd.v23.n4.4997

Keywords:

Ceratitis Capitata, Antifungal Activity, Insecticidal Activity, Achillea millefolium, Chrysanthemum coronarium, Pest Control

Abstract

Of all the insect pests threatening fruit tree cultivation, the Mediterranean fruit fly, Ceratitis capitata, is considered one of the most damaging species, particularly for citrus and peach trees in Mediterranean countries.The main objective of this work is to evaluate the antifungal and insecticidal activity of the essential oils of Achillea millefolium (Asteraceae) and Chrysanthemum coronarium (Asteraceae) against the pests of orange, Citrus sinensis,namely the insect Ceratitis capitata and the pathogenic fungi that accompany it.The essential oils of the two plants used were extracted using a Clevenger-type apparatus. Their antifungal activity was tested by radial culture and their insecticidal activity by fumigation.All essential oils exhibited antifungal activity against Aspergillus niger, Fusarium sp1, Penicillium sp, and Fusarium sp2 at the highest concentration (5 µL/mlair). Achillea millefolium essential oil induced 100% mortality after 24 hours of exposure in adult Ceratitis capitata pests. However, for Chrysanthemum coronarium essential oil, larvae were the most affected stage, reaching approximately 35% mortality at the dose (5 µL/ml air).These essential oils could be used as antifungal and fumigant agents against Ceratitis capitata by increasing the dose.

References

1. Alkan, M., Gökçe, A Tanacetum abrotanifolium(L.) DRUCE (Asteraceae)’un gövdeveçiçekekstraktlarınınSitophilus granariusveSitophilus oryzae (Col., Curculionidae)’ye olankontaktvedavranışsaletkileri. Türkiye EntomolojiDergisi, 36 (3), 377-389. 2012.

2. Alvarez-Castellanos, P. P., Alvarez-Castellanos, C. D., Bishop, M., Pascual-Villalobos, J Antifungal activity of the essential oil of flowerheads of garland chrysanthemum (Chrysanthemum coronarium) against agricultural pathogens. Phytochemistry. 57(1):99‑102. 2001.

3. Bardaweel, S. K., Hudaib M. M., Tawaha, K. A., Bashatwah, R. M Studies on the in vitro antiproliferative, antimicrobial, antioxidant, and acetylcholinesterase inhibition activities associated with Chrysanthemum coronarium essential oil. Evid.-Based Complement.Altern. Med. ECAM. 2015.

4. Barel, S., Segal, R., Yashphe, J The antimicrobial activity of the essential oil from Achillea fragrantissima. Journal of Ethnopharmacology33: 187-191. 1991.

5. Benelli, G Essential oils and their major constituant as noveleco friendly larvicides and adulticides against the Ceratitis capitata. 2018.

6. Benelli, G., Roberto rizzo et al., Carlina acaulis and Trachyspermum ammi essential oils formulated in protein baits are highly toxic and reduce aggressiveness in the medfly, Ceratitis capitataInd. Crops Prod. 2021.

7. Benomari, F. Z Thèse de doctorat LMD. université de Tlemcen .faculté des sciences. Departement de chimie. Variabilité chimique et activités biologiques des volatils des espèces aromatiques à intérêt économique des genres Mentha, Inula, Thymus et Chrysanthemum de l’ouest algérien. pp108. 2018.

8. Bishop, C. D., Pascual-Villalobos, M. J Antifungal activity of the essential oil of flowerheads of garland chrysanthemum (Chrysanthemum coronarium) against agricultural pathogens. Phytochemistry. 57(1):99‑102. 2001.

9. Bounoiura, Y Insecticidal activity of diatomaceous earth on larvae and adults of Ceratitis capitata wied., (Diptera; Tephritidae), citrus pests against ecological control. Journal of Natural Product Research and Applications (JNPRA). Journal of Natural Product Research and Applications; 2024. vol. 4, no 01, p. 11-22. 2024.

10. Caki, A., Hakan, Ö., Tuba, A., Şaban, K., Aysema Tazegül, Ç., Tülay, A., Ebru, M., Adnan, A Phytotoxic and insecticidal properties of essential oils and Extracts of four Achillea species. Rec. Nat. Prod. (2015) 10:2 154-167. 2015.

11. Calmasur, O., Kordali, S., Kaya, O., Aslan, I Toxicity of essential oil vapoursobtained from Achillea spp. to Sitophilus granarius (L.) and Tribolium confusum.Journal of Plant Diseases and Protection113(1): 37-41. 2006.

12. Féron, M L’instinct de reproduction chez la mouche méditerranéenne des fruits (Ceratitis capitata Wiedemann) (Diptera : Tephritidae). Comportement sexuel. Comportement de ponte. Revue de Pathologie végétale et d’entomologie Agricole de France, 41. pp. 1-129. 1962.

13. Flamini, P., Cioni, L., Morelli, I Differences in the fragrances of pollen, leaves, and floral parts of garland (Chrysanthemum coronarium) and composition of the essential oils from flowerheads and leaves. Agric. Food Chem. 51(8):2267–2271. 2003.

14. Gökce, A., Whalon, M.E., Cam, H., Yanar, Y., Demirtas, I., Gören, N Contact and Residual Toxicities of 30 Plant Extracts to Colorado Potato Beetle Larvae. Archives of Phytopathology and Plant Protection, 149(2), 1-10. 2007.

15. Iannacone, J., Lamas, G Effecto insecticida de cuatro extractosbotánicos y delcartap sobre la polilla de la papa Phthorimaeaoperculella (Zeller) (Lepidoptera:Gelechiidae), en el Perú. 2003.

16. Istamkulova, M. M., Mukhamadiev, A. N., Mukhamadiev, N. K., Keldiyarov, H. O., Istamkulov, M. S GC-MS Analysis of Essential Oils of Species of the Genus Achillea L. Am J Biomed Sci & Res. 24(1) 2024. AJBSR.MS.ID.003160.

17. Jovanovic, Z., Kosti, M., Popovic, Z Grain-protective properties of herbal extract against the bean weevil AcanthoscelidesobtectusSay. Industrial Crops and Products 26: 100-104. 2007.

18. Khani, A., Asghari, J Insecticide activity of essential oils of Mentha longifolia, Pulicariagnaphalodesand Achillea wilhelmsiiagainst two stored product pests, the flour beetle, Tribolium castaneum, and the cowpea weevil, Callosobruchus maculatus. Journal of Insect Science 12:73. 2012.

19. Kim, S.I., Park, C., Ohh, M.H., Cho, H.C., Ahn, Y.J Contact and fumigant activities of aromatic plant extracts and essential oils against Lasioderma serricorne (Coleoptera: Anobiidae). Journal of Stored Products Research, 39(1), 11-19. 2003.

20. Kordali, S., Memis, K., Cakir, A Toxicity of monoterpenes against larvae and adults of Colorado potato beetle, Leptinotarsa decemlineataSay (Coleoptera: Chrysomelidae). Industrial Crops and Products, 26, 278-297. 2007.

21. Magdy, I. E. M., Samir, A. M. A Chemical composition and insecticidal potential of essential oils from Egyptian plants against Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Triboliumcastaneum (Herbst) (Coleoptera: Tenebrionidae). Applied Entomology and Zoology 43(4): 599-607. 2008.

22. Magiatis, P., Skaltsounis, A.L., Chinov, I., Haroutounian, S.A Chemical composition and in vitro antimicrobial activity of the essential oils of three Greek Achillea species. Zeitschrift für Naturforschung57:287-290. 2002.

23. Mohamed-Elamir, F., Ahmed, A., Amira, M. G., Turky, F., Toshifumi, H., Paul, W., Parée, J. K., Mohamed, S. K., Ahmed A Anti-inflammatory Activity of New Guaiane Acid Derivatives from Achillea Compacta, NPC 851-856. 2008.

24. Motavaliza dehkakhk, A., Shafaghat, A., Zamani, H., Akhlaghi, H., Hosseini, M Compositions and the in vitro antimicrobial activities of the essential oils and extracts of two Achillea species from Iran 2013, journal of Medicinal plants research. 2013.

25. Perez, M. P. al., Effects of the essential oil of flower heads of Chrysanthemum coronarium L. on white fly and stored product pests. Investig. Agrar. Prod. Prot. Veg. Espana. 1999.

26. Pérez, M.P., Navas-Cortés, J. A., Pascual-Villalobos, M. J., Castillo, P Nematicidal activity of essential oils and organic amendments from Asteraceae against root-knot nematodes. Plant Pathol. 52(3):395‑401. 2003.

27. Polatoğlu K., Karakoç Ö.C., Gören N Phytotoxic. DPPH scavenging. Insecticidactivities and essential oil composition of Achillea vermicularis. A. teretifoliaand proposed chemotypes of A. biebersteinii(Asteraceae). Industrial crops and products, 51, 35-45. 2013.

28. Rossi, Y.F Fumigant and contact insecticidal activity of five essential oils against Ceratitis capitata. 2012.

29. Tabti, L Comparaison des activités insecticide et antimicrobienne de Thymus capitatus(L.) Hoffm. & Link, Tetraclinisarticulata (Vahl.)Masters et Daucus crinitusDesf. contre les pathogènes de l’oranger Citrus sinensis dans la région de Tlemcen. Thèse de doctorat, 143pp. Université de Tlemcen. 2015.

30. Wiedemann, C.R.W Munusrectoris in Acadamia Christiana Albertina aditurus Analecta entomologica ex Museo Regio Havniesimaximecongesta profert inconibusqueillustrat. 60p. kiel. 1829.

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Published

2026-02-23

How to Cite

Zohra, B. F., Wahiba, K., Amine, D. M. E., Yassine, B., & Nassira, G. B. (2026). ANTIFUNGAL AND INSECTICIDAL ACTIVITIES OF ESSENTIAL OILS OF CHRYSANTHEMUM CORONARIUM AND ACHILLEA MILLEFOLIUM AGAINST CITRUS FRUITS PESTS AND DISEASES. Veredas Do Direito, 23, e234997. https://doi.org/10.18623/rvd.v23.n4.4997